A simple, precise, accurate, reproducible First order derivative Spectroscopic method was developed for the simultaneous estimation of Montelukast Sodium and Olopatadine Hydrochloride in Combined dosage form using methanol. In this study the zero-crossing technique was used for spectral interpretaion. The method was found to be linear in the range of 10-50 μg/ml (r2=0.9994) for Montelukast sodium at 340.2 nm (Zero crossing point of Olopatadine Hydrochloride) and the range of 5-25 μg/ml (r2=0.9992) for Olopatadine Hydrochloride at 315.6 nm (Zero crossing point of Montelukast Sodium). The proposed method was validated for linearity, precision, accuracy and ruggedness according to ICH guideline for routine estimation purpose of these drugs in combined bulk dosage form. Since the 1st derivative method has been developed and validated to ensure that the performance characteristic of the method meets the requirements for the intended analytical applications.
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